Surprisingly, MS patients showed a higher diversity of TCR repertoires than other groups. In addition, longitudinal analysis of TCR repertoires from HAM/TSP patients demonstrated a correlation of the TCR clonal expansion with HTLV-I proviral load. While HAM/TSP patients showed a higher clonal T-cell expansion compared to MS and HC, increase of the TCR clonal expansion was inversely correlated with the diversity of TCR repertoire in all subjects. cDNA-TCR β-chain libraries were sequenced from 2 million peripheral mononuclear cells (PBMCs) in 14 HAM/TSP patients, 34 MS patients and 20 healthy controls (HC). In this study, we applied an unbiased molecular technique – unique molecular identifier (UMI) library-based strategy to investigate with high accuracy the TCR clonal repertoire by high throughput sequencing (HTS) technology. We hypothesized that a T-cell receptor (TCR) clonal repertoire ‘signature’ could distinguish HAM/TSP patients from healthy controls, as well as from patients with a more heterogeneous CNS-reactive inflammatory disease such as MS. 2011 12:2825–30.In this study we characterized the TCR repertoire profiles in patients with chronic progressive inflammatory neurological disorders including HAM/TSP, associated with human T-cell lymphotropic virus type I (HTLV-I) infection, and multiple sclerosis (MS), an inflammatory, demyelinating disease of the CNS of unknown etiology. Scikit-learn: machine learning in python. Pedregosa F, Varoquaux G, Gramfort A, Michel V, Thirion B, Grisel O, et al. Multidisciplinary study of the secondary immune response in grandparents re-exposed to chickenpox. Ogunjimi B, Van den Bergh J, Meysman P, Heynderickx S, Bergs K, Jansen H, et al. CMV induces expansion of highly polyfunctional CD4 + T cell subset coexpressing CD57 and CD154. Pera A, Vasudev A, Tan C, Kared H, Solana R, Larbi A. Human Cytomegalovirus (HCMV)-Specific CD4 + T Cells are polyfunctional and can respond to HCMV-infected dendritic cells.
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High-throughput sequencing of TCR repertoires in multiple sclerosis reveals intrathecal enrichment of EBV-reactive CD8 + T cells. Lossius A, Johansen JN, Vartdal F, Robins H, Šaltyte BJ, Holmøy T, et al. Skewed T cell receptor repertoire in genetically identical twins correlates with multiple sclerosis. Utz U, Biddison WE, McFarland HF, McFarlin DE, Flerlage M, Martin R. Beta-Cell-specific CD8 T cell phenotype in type 1 diabetes reflects chronic autoantigen exposure. Skowera A, Ladell K, McLaren JE, Dolton G, Matthews KK, Gostick E, et al. On the feasibility of mining CD8 + T cell receptor patterns underlying immunogenic peptide recognition. 2017 17:61.ĭe Neuter N, Bittremieux W, Beirnaert C, Cuypers B, Mrzic A, Moris P, et al. Overview of methodologies for T-cell receptor repertoire analysis. Rosati E, Dowds CM, Liaskou E, Henriksen EKK, Karlsen TH, Franke A. A bioinformatic framework for immune repertoire diversity profiling enables detection of immunological status.
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